The most cost effective route is: Cincinnati - Winchester - Knoxville - Cleveland - Dalton - Cartersville - Atlanta - Macon - Jesup. You're going to hit the most population for the lowest cost.
[/QUOTE
Why Cleveland and Dalton with no stop in Chattanooga? Potential passengers from the Dalton/Cleveland area could easily get to Chattanooga to board the train.
I don't want to bypass Knoxville, that's why I mentioned routing the train through Knoxville... it's just out of the way. Would be more direct to stay on the main through Dayton and go through Chattanooga. It doesn't make sense to hit both Knox and Chatt directly, so pick the one that makes the most sense time-wise. Chattanooga and Knoxville are almost identical population-wise.
I support your thinking. One reason for including Knoxville would be to hopefully obtain some patronage from the students attending the University of Tennessee.
However, such considerations don't seem to be attractive to Amtrak's schedulers. The Cardinal goes through Oxford, Ohio--the home home of Miami University--without making even a flag stop. Over the years, there has been efforts made to get a flag stop in that community with no success.